4F8Q
Crystal Structure of the Human BTN3A2 Ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 33.001, 77.579, 100.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.313 - 2.379 |
R-factor | 0.2339 |
Rwork | 0.232 |
R-free | 0.26850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f80 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.321 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.420 |
High resolution limit [Å] | 2.379 | 6.460 | 2.379 |
Number of reflections | 10267 | ||
Completeness [%] | 93.2 | 98.3 | 50 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.1M Sodium Citrate pH5.6, 0.5M Ammonium Sulfate, 1M Lithium Sulfate, vapor diffusion, sitting drop, temperature 298K |