4F8Q

Crystal Structure of the Human BTN3A2 Ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 2 21
Unit cell lengths	33.001, 77.579, 100.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.313 - 2.379
R-factor	0.2339
Rwork	0.232
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f80
RMSD bond length	0.012
RMSD bond angle	1.321
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.420
High resolution limit [Å]	2.379	6.460	2.379
Number of reflections	10267
Completeness [%]	93.2	98.3	50

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	0.1M Sodium Citrate pH5.6, 0.5M Ammonium Sulfate, 1M Lithium Sulfate, vapor diffusion, sitting drop, temperature 298K