4F8F
Human Insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.120, 82.120, 33.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.060 - 1.676 |
| R-factor | 0.1688 |
| Rwork | 0.167 |
| R-free | 0.19770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.176 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.060 | 30.528 | 1.760 |
| High resolution limit [Å] | 1.676 | 5.280 | 1.676 |
| Rmerge | 0.056 | 0.224 | |
| Total number of observations | 1916 | 6810 | |
| Number of reflections | 9692 | ||
| <I/σ(I)> | 17.6 | 7.7 | 3.3 |
| Completeness [%] | 98.1 | 99.6 | 87.3 |
| Redundancy | 5.9 | 6.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 2 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Humulin R, lot # A 405936), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
