4F85
Structure analysis of Geranyl diphosphate methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 143.938, 143.938, 66.724 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.490 - 2.200 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bus |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.421 |
Number of reflections | 21196 | |
<I/σ(I)> | 33.9 | 4.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 15 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 293 | 1M ammonium suflate, 8% glycerol, 0.1M Tris-HCl, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |