4F7K
Crystal structure of Lac15 from a marine microbial metagenome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-01 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97916 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.410, 91.360, 86.150 |
Unit cell angles | 90.00, 112.10, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.21687 |
Rwork | 0.214 |
R-free | 0.26534 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yxw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.461 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 | |
Number of reflections | 41974 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 98.4 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M MES, 18% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |