4F7H

The crystal structure of kindlin-2 pleckstrin homology domain in free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-07
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	58.474, 58.474, 92.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.237 - 1.900
R-factor	0.1964
Rwork	0.195
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ys3
RMSD bond length	0.013
RMSD bond angle	1.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.237	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	13245
Completeness [%]	99.9	100
Redundancy	9.4	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	1.4 M ammonium tartrate dibasic, 0.1 M tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K