4F7F
Structure of Anopheles gambiae odorant binding protein 20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.794, 36.374, 96.154 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 24.033 - 1.800 |
| R-factor | 0.1982 |
| Rwork | 0.195 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QDI |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.276 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.062 | 0.380 |
| Number of reflections | 48493 | |
| <I/σ(I)> | 8.5 | 2.3 |
| Completeness [%] | 88.3 | 94 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.57 | 276 | 0.2 M potassium sodium tartrate, 0.1 M sodium citrate, 1.9 M ammonium sulfate, pH 5.57, VAPOR DIFFUSION, HANGING DROP, temperature 276K |
