4F7D

Crystal structure of ferredoxin-NADP reductase from burkholderia thailandensis E264

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-04
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	70.020, 85.570, 102.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.140 - 2.350
R-factor	0.206
Rwork	0.204
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.315
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.140	2.410
High resolution limit [Å]	2.350	2.350
Rmerge	0.074	0.479
Number of reflections	26192
<I/σ(I)>	20.49	4.4
Completeness [%]	99.7	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	EBS INTERNAL TRACKING NUMBER HEPES (PH 7.0), 500 MM NACL, 2 MM DTT, 0.025% SODIUM AZIDE, 5% GLYCEROL, 0.4 UL X 0.4 UL DROP WITH 20% PEG 3350, 200 MM NACL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K