4F7A

Crystal structure of a susd homolog (BVU_2203) from Bacteroides vulgatus ATCC 8482 at 1.85 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-09
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 43 3 2
Unit cell lengths	160.379, 160.379, 160.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.782 - 1.850
R-factor	0.1423
Rwork	0.141
R-free	0.16160
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.276
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.782	29.782	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge		0.055	0.916
Total number of observations		7204	48108
Number of reflections	60397
<I/σ(I)>	11.4	11.1	0.8
Completeness [%]	99.9	96.2	100
Redundancy	10.9	9.4	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.17M ammonium acetate, 15.0% Glycerol, 25.5% polyethylene glycol 4000, 0.1M sodium citrate pH 5.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K