4F7A
Crystal structure of a susd homolog (BVU_2203) from Bacteroides vulgatus ATCC 8482 at 1.85 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-09 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 160.379, 160.379, 160.379 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.782 - 1.850 |
R-factor | 0.1423 |
Rwork | 0.141 |
R-free | 0.16160 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.276 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.782 | 29.782 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.055 | 0.916 | |
Total number of observations | 7204 | 48108 | |
Number of reflections | 60397 | ||
<I/σ(I)> | 11.4 | 11.1 | 0.8 |
Completeness [%] | 99.9 | 96.2 | 100 |
Redundancy | 10.9 | 9.4 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.17M ammonium acetate, 15.0% Glycerol, 25.5% polyethylene glycol 4000, 0.1M sodium citrate pH 5.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |