4F6M
Crystal structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.2819 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 44.760, 185.180, 112.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.690 - 2.400 |
| R-factor | 0.22306 |
| Rwork | 0.222 |
| R-free | 0.23986 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.103 | 0.515 |
| Number of reflections | 18774 | |
| <I/σ(I)> | 11.4 | 1.8 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.67 | 4.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2M sodium nitrate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
