4F6A
High-resolution x-ray Structure of the tetramutant of BH1408 protein from Bacillus halodurans, Northeast Structural Genomics Consortium (NESG) Target BhR182
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-05 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97907 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 86.842, 86.842, 163.818 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.174 - 2.009 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.21970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e0a |
RMSD bond length | 0.008 |
RMSD bond angle | 1.002 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (dev_988) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.072 |
Number of reflections | 46521 |
<I/σ(I)> | 33.1 |
Completeness [%] | 92.6 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1M KSCN, 0.1M TAPS, 20% PEG 4K, VAPOR DIFFUSION, HANGING DROP, temperature 277K |