4F5U
Crystal structure of Equine Serum Albumin at 2.04 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9320 |
| Spacegroup name | P 61 |
| Unit cell lengths | 89.254, 89.254, 134.802 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.040 |
| R-factor | 0.19756 |
| Rwork | 0.196 |
| R-free | 0.24294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f5t |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.966 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.110 | 0.639 |
| Number of reflections | 38668 | |
| <I/σ(I)> | 16.7 | 2.1 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 80% Tacsimate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
