4F5F

Structure of Aspartate Aminotransferase Conversion to Tyrosine Aminotransferase: Chimera P1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	85
Detector technology	CCD
Collection date	2010-08-18
Detector	BRUKER SMART 6000
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	60.189, 103.685, 138.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.703 - 2.250
R-factor	0.1761
Rwork	0.174
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.045
Data reduction software	PROTEUM PLUS (PLUS)
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.703	2.340
High resolution limit [Å]	2.250	2.250
Rmerge	0.087	0.325
Number of reflections	41556
<I/σ(I)>	12.83	2.53
Completeness [%]	98.6	87.5
Redundancy	4.91	2.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	8% PEG 4000, 0.1 M Sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K