4F57
Fab structure of a neutralizing antibody L1 from an early subtype A HIV-1 infected patient
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-27 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03319 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.101, 57.463, 60.038 |
| Unit cell angles | 90.00, 112.78, 90.00 |
Refinement procedure
| Resolution | 41.500 - 1.700 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nh7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 50571 | |
| Completeness [%] | 95.8 | 81.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 0.17 M (NH4)2SO4, 0.085 M cacodylate pH 6.5, 25.5% (w/v) polyethylene glycol (PEG) 8000, and 15% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
