4F4Z
Y-family DNA polymerase chimera Dpo4-Dpo4-Dbh
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 50.966, 51.196, 98.842 |
Unit cell angles | 75.60, 82.97, 70.14 |
Refinement procedure
Resolution | 27.065 - 2.305 |
R-factor | 0.1883 |
Rwork | 0.185 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.142 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (in PHENIX) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.030 | 0.190 |
Number of reflections | 21042 | |
<I/σ(I)> | 21.3 | 2 |
Completeness [%] | 81.1 | 50.5 |
Redundancy | 1.8 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10% PEG-3350, 100 mM Ca(OAc)2, 100 mM HEPES, 2.5% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |