4F4M

Structure of the type VI peptidoglycan amidase effector Tse1 (C30A) from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	40.018, 108.857, 82.531
Unit cell angles	90.00, 93.46, 90.00

Refinement procedure

Resolution	65.690 - 2.677
R-factor	0.2056
Rwork	0.203
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (Phaser)
Refinement software	PHENIX ((phenix.refine: dev_1047))

Data quality characteristics

	Overall
Low resolution limit [Å]	65.690
High resolution limit [Å]	2.677
Rmerge	0.136
Number of reflections	19072
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	Crystals were obtained by sitting drop vapor diffusion at 25 oC from a 1:1 mixture of 10 mg/mL protein with 0.2 M sodium thiocyanate, 0.1 M HEPES (pH 7.5), 20% PEG3350 for 3 days. Crystals were immersed in mother liquor containing 20% glycerol, mounted, and flash frozen in liquid N2. , VAPOR DIFFUSION, HANGING DROP, temperature 297K