4F3M

Crystal structure of CRISPR-associated protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9770
Spacegroup name	C 1 2 1
Unit cell lengths	86.509, 46.687, 129.030
Unit cell angles	90.00, 104.01, 90.00

Refinement procedure

Resolution	20.000 - 1.710
R-factor	0.1565
Rwork	0.152
R-free	0.23010
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.685
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	50063
Completeness [%]	91.8	76.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.5	0.1 M MES, 0.8 M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K
1	VAPOR DIFFUSION, HANGING DROP	6	291.5	0.1 M MES, 0.8 M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K
1	VAPOR DIFFUSION, HANGING DROP	6	291.5	0.1 M MES, 0.8 M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K