4F3H

The structural of FimXEAL-c-di-GMP from Xanthomonas campestris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	65.665, 65.665, 121.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.260 - 2.500
R-factor	0.234
Rwork	0.234
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f48
RMSD bond length	0.076
RMSD bond angle	5.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500	2.500
Number of reflections	10851
Completeness [%]	98.7	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	20% PEG 3350, 0.2M Sodium Formate (pH7.2), pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K