4F3E
Crystal Structure of Thermus thermophilus HB8 CasA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.888, 47.926, 129.168 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 45.703 - 2.400 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.25100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.058 | 2.280 |
| High resolution limit [Å] | 1.984 | 2.200 |
| Rmerge | 0.101 | 1.000 |
| Number of reflections | 57797 | |
| <I/σ(I)> | 11.162 | 1.025 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 300 | 2.3 M sodium acetate, 0.1 M MOPS, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
