4F30
Structure of RPE65: P6522 crystal form grown in ammonium phosphate solution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 178.497, 178.497, 86.642 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.440 - 3.150 |
R-factor | 0.21658 |
Rwork | 0.215 |
R-free | 0.24707 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4f3a |
RMSD bond length | 0.005 |
RMSD bond angle | 1.006 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.350 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.108 | 0.495 |
Number of reflections | 14356 | |
<I/σ(I)> | 12.1 | 2.78 |
Completeness [%] | 99.0 | 98.6 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 1 M ammonium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |