4F30
Structure of RPE65: P6522 crystal form grown in ammonium phosphate solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 178.497, 178.497, 86.642 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.440 - 3.150 |
| R-factor | 0.21658 |
| Rwork | 0.215 |
| R-free | 0.24707 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4f3a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.108 | 0.495 |
| Number of reflections | 14356 | |
| <I/σ(I)> | 12.1 | 2.78 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 1 M ammonium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
