4F2R
DNA Polymerase I Large Fragment complex 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Collection date | 2011-10-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.220, 108.480, 150.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.980 - 1.630 |
R-factor | 0.1708 |
Rwork | 0.170 |
R-free | 0.19240 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2HVI WITH INSERTION SITE BASE PAIR METAL IONS AND PROTEIN RESIDUES 681-721 DELETED |
RMSD bond length | 0.011 |
RMSD bond angle | 1.375 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (dev_1026) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.630 | 4.870 | 1.630 |
Rmerge | 0.063 | 0.036 | 0.506 |
Number of reflections | 190429 | 7543 | 30817 |
<I/σ(I)> | 20.25 | 50.81 | 3.38 |
Completeness [%] | 100.0 | 99.6 | 99.8 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 47%-49% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MgSO4, 100mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |