4F2Q
Quisqualate bound to the D655A mutant of the ligand binding domain of GluA3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 47.660, 48.027, 136.861 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.659 - 2.202 |
R-factor | 0.2014 |
Rwork | 0.195 |
R-free | 0.26090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dln |
RMSD bond length | 0.013 |
RMSD bond angle | 1.466 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.189 | 0.758 |
<I/σ(I)> | 10.406 | 2.194 |
Completeness [%] | 99.0 | 100 |
Redundancy | 5.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 14-15% PEG 8K, 0.1 M sodium cacodylate, 0.1-0.15 M zinc acetate, 0.25 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |