4F2J

Crystal structure of ZNF217 bound to DNA, P6522 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	56.928, 56.928, 242.542
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.310 - 2.640
R-factor	0.2529
Rwork	0.252
R-free	0.26430
Structure solution method	SIRAS
RMSD bond length	0.016
RMSD bond angle	2.318
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.000	99.000	2.680
High resolution limit [Å]	2.640	7.160	2.640
Rmerge	0.091	0.108	0.188
Number of reflections	7517
<I/σ(I)>	9.3
Completeness [%]	98.5	94	95
Redundancy	2.5	2.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 3350, 0.2M Sodium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
2	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 3350, 0.2M Sodium sulfate, 10mM Trimethyllead acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K