4F2H

Structure of the minimal Ste5 VWA domain subject to autoinhibition by the Ste5 PH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	63.840, 87.350, 100.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.694 - 3.192
R-factor	0.1869
Rwork	0.183
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fze
RMSD bond length	0.008
RMSD bond angle	1.982
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	3.280
High resolution limit [Å]	3.190	3.190
Number of reflections	4395
<I/σ(I)>	25.4	10.56
Completeness [%]	90.0	89.9
Redundancy	5.75	4.91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	20% PEG3350, 0.1 M calcium chloride, 25 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	8	290	20% PEG3350, 0.1 M calcium chloride, 25 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K