4F28

The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	41.330, 56.600, 59.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.870 - 1.550
R-factor	0.2177
Rwork	0.216
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.870		1.610
High resolution limit [Å]	1.550	3.330	1.550
Rmerge	0.047	0.034	0.991
Number of reflections	20457	2223	1899
<I/σ(I)>	25.26	67.28	1.56
Completeness [%]	98.4	98.2	87.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1