4F1V
Subatomic resolution structure of a high affinity periplasmic phosphate-binding protein (PfluDING) bound with phosphate at pH 8.5
Replaces: 3G63Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Detector technology | CCD |
Collection date | 2007-02-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.75 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.960, 124.900, 40.920 |
Unit cell angles | 90.00, 116.31, 90.00 |
Refinement procedure
Resolution | 62.500 - 0.880 |
R-factor | 0.126 |
Rwork | 0.125 |
R-free | 0.13981 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3G63 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.056 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 62.500 |
High resolution limit [Å] | 0.880 |
Number of reflections | 254891 |
Completeness [%] | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |