4F1V
Subatomic resolution structure of a high affinity periplasmic phosphate-binding protein (PfluDING) bound with phosphate at pH 8.5
Replaces: 3G63Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | CCD |
| Collection date | 2007-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.75 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.960, 124.900, 40.920 |
| Unit cell angles | 90.00, 116.31, 90.00 |
Refinement procedure
| Resolution | 62.500 - 0.880 |
| R-factor | 0.126 |
| Rwork | 0.125 |
| R-free | 0.13981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3G63 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.056 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 62.500 |
| High resolution limit [Å] | 0.880 |
| Number of reflections | 254891 |
| Completeness [%] | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
