4F1N
Crystal structure of Kluyveromyces polysporus Argonaute with a guide RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 32 |
Unit cell lengths | 171.553, 171.553, 83.177 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.119 - 3.187 |
R-factor | 0.1705 |
Rwork | 0.168 |
R-free | 0.21620 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.347 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.187 |
Rmerge | 0.186 |
Number of reflections | 45536 |
<I/σ(I)> | 12.8 |
Completeness [%] | 100.0 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION, RECRYSTALLIZATION | 5 | 293 | 100 mM MIB pH 5.0, 3% 1,4-dioxane, 19% PEG3350, 12 mM MnCl2, and 3% ethanol, EVAPORATION, RECRYSTALLIZATION, temperature 293K |