4F1N

Crystal structure of Kluyveromyces polysporus Argonaute with a guide RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	293
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 32
Unit cell lengths	171.553, 171.553, 83.177
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.119 - 3.187
R-factor	0.1705
Rwork	0.168
R-free	0.21620
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.347
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.187
Rmerge	0.186
Number of reflections	45536
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION	5	293	100 mM MIB pH 5.0, 3% 1,4-dioxane, 19% PEG3350, 12 mM MnCl2, and 3% ethanol, EVAPORATION, RECRYSTALLIZATION, temperature 293K