4F1J
Crystal structure of the MG2+ loaded VWA domain of plasmodium falciparum trap protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 89.313, 89.313, 104.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.730 |
R-factor | 0.17905 |
Rwork | 0.177 |
R-free | 0.21819 |
Structure solution method | SIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.399 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.130 | 0.853 |
<I/σ(I)> | 14.72 | 2.9 |
Completeness [%] | 99.8 | 98.6 |
Redundancy | 10.271 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG 3350, 0.1M BIS-TRIS, 0.2M MAGNESIUM SULFATE, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |