4F11

Crystal structure of the extracellular domain of human GABA(B) receptor GBR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0715
Spacegroup name	C 1 2 1
Unit cell lengths	153.212, 92.716, 48.558
Unit cell angles	90.00, 102.75, 90.00

Refinement procedure

Resolution	44.710 - 2.380
R-factor	0.2017
Rwork	0.201
R-free	0.21710
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.380	5.170	2.380
Rmerge	0.049	0.034	0.411
Number of reflections	25223
<I/σ(I)>	27.4
Completeness [%]	96.3	95.7	75.6
Redundancy	3.5	3.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	18% PEG MME 550, 20% glycerol, 2mM zinc sulfate, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K