4F0V
Crystal structure of type effector Tse1 from Pseudomonas aeruginousa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.265, 61.723, 74.094 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.522 - 1.600 |
R-factor | 0.1566 |
Rwork | 0.156 |
R-free | 0.17210 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.7.3_928) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.085 | 0.373 |
Number of reflections | 23887 | |
<I/σ(I)> | 67.5 | 11.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.8 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Succinic acid pH 7.0, 15% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |