4F0U

X-Ray Crystal Structure of Allophycocyanin from Synechococcus elongatus PCC 7942

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	C 2 2 21
Unit cell lengths	99.550, 165.330, 151.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.500
Rwork	0.235
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.081	0.455
Number of reflections	42702
<I/σ(I)>	9.3	2.3
Completeness [%]	98.8	98.9
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	30% PEG 4000; 50mM Tris pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K