4F0T

X-Ray Crystal Structure of Phycocyanin from Synechocystis sp. PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 63
Unit cell lengths	153.020, 153.020, 40.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.250
R-factor	0.21784
Rwork	0.216
R-free	0.24992
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.084	0.310
Number of reflections	25948
<I/σ(I)>	25.7	7.7
Completeness [%]	100.0	100
Redundancy	7.8	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	6% PEG 4000; 400mM MgSO4; 20mM Tris pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K