4F03
Crystal structure of the glutathione transferase GTE1 from Phanerochaete chrysosporium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.989, 88.383, 157.007 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.539 - 1.800 |
R-factor | 0.1926 |
Rwork | 0.192 |
R-free | 0.22700 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.025 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.230 | 1.890 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.619 |
Number of reflections | 111495 | |
<I/σ(I)> | 22.2 | 3.6 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 277 | 30% PEG 8000, 200mM sodium acetate, 100 mM sodium cacodylate, pH 6.5, MICROBATCH, temperature 277K |