4EZZ
Crystal structure of the substrate binding domain of E.coli DnaK in complex with the designer peptide ELPLVKI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 93.711, 116.593, 37.056 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.050 |
R-factor | 0.22135 |
Rwork | 0.218 |
R-free | 0.30779 |
Structure solution method | PDB ID 1DKZ |
RMSD bond length | 0.016 |
RMSD bond angle | 1.881 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC (with PDBID 1DKZ) |
Refinement software | REFMAC (with PDBID 1DKZ) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.053 | 0.475 |
Number of reflections | 12888 | |
Completeness [%] | 98.1 | 94.3 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 292 | 3.0 M ammonium sulfate, 0.1 M bicine pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |