4EZY
Crystal structure of the substrate binding domain of E.coli DnaK in complex with the designer peptide NRLILTG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 94.435, 116.824, 37.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.850 |
| R-factor | 0.20518 |
| Rwork | 0.202 |
| R-free | 0.27977 |
| Structure solution method | PDB ID 1DKZ |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.929 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC (with PDBID 1DKZ) |
| Refinement software | REFMAC (with PDBID 1DKZ) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.051 | 0.474 |
| Number of reflections | 18035 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 3.1 M ammonium sulfate, 0.1 M MES pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
