4EZW
Crystal structure of the substrate binding domain of E.coli DnaK in complex with the designer peptide NRLLLTG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99188 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 157.124, 78.026, 89.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.700 - 1.800 |
R-factor | 0.19361 |
Rwork | 0.193 |
R-free | 0.22628 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 2.106 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.044 | 0.455 |
Number of reflections | 102300 | |
Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 292 | 2.4 M ammonium sulfate, 0.1 M citric acid pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 292K |