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4EZU

Crystal structure of the substrate binding domain of E.coli DnaK in complex with PR-bombesin in space group I222

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.2
Synchrotron siteBESSY
Beamline14.2
Temperature [K]100
Detector technologyCCD
Collection date2010-10-19
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.91841
Spacegroup nameI 2 2 2
Unit cell lengths96.645, 115.047, 39.366
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution25.000 - 1.900
R-factor0.22064
Rwork0.218
R-free0.27582
Structure solution methodPDB ID 1DKZ
RMSD bond length0.017
RMSD bond angle1.931
Data reduction softwareXDS
Data scaling softwareSCALA
Phasing softwareREFMAC (with PDBID 1DKZ)
Refinement softwareREFMAC (with PDBID 1DKZ)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0002.000
High resolution limit [Å]1.9001.900
Rmerge0.0620.558
Number of reflections17741
Completeness [%]99.8100
Redundancy5.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82923.0 M ammonium sulfate, 0.1 M HEPES pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K

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