4EZU
Crystal structure of the substrate binding domain of E.coli DnaK in complex with PR-bombesin in space group I222
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 96.645, 115.047, 39.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.900 |
R-factor | 0.22064 |
Rwork | 0.218 |
R-free | 0.27582 |
Structure solution method | PDB ID 1DKZ |
RMSD bond length | 0.017 |
RMSD bond angle | 1.931 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC (with PDBID 1DKZ) |
Refinement software | REFMAC (with PDBID 1DKZ) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.062 | 0.558 |
Number of reflections | 17741 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 3.0 M ammonium sulfate, 0.1 M HEPES pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |