4EZP
Crystal structure of the substrate binding domain of E.coli DnaK in complex with A3-APO(residues 1 to 20)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.681, 160.924, 44.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.800 - 1.650 |
R-factor | 0.2042 |
Rwork | 0.203 |
R-free | 0.24212 |
Structure solution method | PDB ENTRY 3DPO |
RMSD bond length | 0.019 |
RMSD bond angle | 1.952 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC (with 3DPO) |
Refinement software | REFMAC (with 3DPO) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.045 | 0.479 |
Number of reflections | 65834 | |
Completeness [%] | 96.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 2.4 M ammonium sulfate, 0.1 M sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |