4EZO
Crystal structure of the substrate binding domain of E.coli DnaK in complex with PR-39 (residues 1 to 15)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.632, 161.268, 44.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.800 - 1.900 |
R-factor | 0.20552 |
Rwork | 0.204 |
R-free | 0.23963 |
Structure solution method | PDB ENTRY 3DPO |
RMSD bond length | 0.019 |
RMSD bond angle | 2.024 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC (with PDBID 3DPO) |
Refinement software | REFMAC (with PDBID 3DPO) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.800 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.042 | 0.457 |
Number of reflections | 45171 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 292 | 2.4 M ammonium sulfate, 0.1 M citric acid pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |