4EZG

Crystal structure of a putative cell adhesion protein (LMOf2365_1307) from Listeria monocytogenes str. 4b F2365 at 1.50 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-10
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	59.696, 62.979, 107.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.754 - 1.500
R-factor	0.1717
Rwork	0.171
R-free	0.19290
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.161
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	PHENIX (1.7.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.754	28.754	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.046	0.022	0.630
Number of reflections	64777	12122	11354
<I/σ(I)>	13.87	40.1	2
Completeness [%]	98.0	96.4	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.17M ammonium sulfate, 15.00% Glycerol, 25.50% polyethylene glycol 4000, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K