4EZC
Crystal Structure of the UT-B Urea Transporter from Bos Taurus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-01-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.703, 105.645, 105.870 |
| Unit cell angles | 90.00, 99.00, 90.00 |
Refinement procedure
| Resolution | 42.950 - 2.360 |
| R-factor | 0.1973 |
| Rwork | 0.196 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.417 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.092 | 0.050 | 0.585 |
| Number of reflections | 66569 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 99.3 | 97.3 | 93.4 |
| Redundancy | 3.6 | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 25% PEG 400, 50 mM sodium sulfate, 50 mM lithium sulfate, 0.2 mM DDAO, 100 mM Tris pH 8.0-8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
