4EZB
CRYSTAL STRUCTURE OF the Conserved hypothetical protein from Sinorhizobium meliloti 1021
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.312, 51.264, 51.068 |
| Unit cell angles | 90.00, 105.44, 90.00 |
Refinement procedure
| Resolution | 19.090 - 2.100 |
| R-factor | 0.1957 |
| Rwork | 0.193 |
| R-free | 0.24420 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.086 | 0.040 | 0.675 |
| Number of reflections | 30844 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 94.1 | 99.3 | 56.5 |
| Redundancy | 3.2 | 3.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M MgCl2, 0.1M Tris-HCl, pH 8.5, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
