4EZ9

Bacillus DNA Polymerase I Large Fragment Complex 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Detector technology	CCD
Collection date	2010-04-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	93.920, 108.960, 149.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.125 - 1.640
R-factor	0.1691
Rwork	0.168
R-free	0.19090
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2HVI WITH INSERTION SITE BASE PAIR METAL IONS AND PROTEIN RESIDUES 681-721 DELETED
RMSD bond length	0.010
RMSD bond angle	1.348
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (dev_1026)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.740
High resolution limit [Å]	1.640	4.880	1.640
Rmerge	0.042	0.018	0.496
Number of reflections	171000
<I/σ(I)>	22.9	68.78	3.85
Completeness [%]	91.2	92.5	99.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	290	48%-53% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MgSO4, 100mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K