4EYZ

Crystal structure of an uncommon cellulosome-related protein module from Ruminococcus flavefaciens that resembles papain-like cysteine peptidases

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	65.811, 60.605, 66.126
Unit cell angles	90.00, 107.66, 90.00

Refinement procedure

Resolution	38.935 - 1.383
R-factor	0.1271
Rwork	0.127
R-free	0.15240
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.195
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX (dev_1180)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.400
High resolution limit [Å]	1.380	3.750	1.380
Rmerge	0.051	0.026	0.449
Number of reflections	182049
<I/σ(I)>	15.4
Completeness [%]	95.9	96.1	83.5
Redundancy	7.3	7.2	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.2M ammonium acetate, 0.1M BIS-TRIS and 25% w/v polyethylene glycol 3350, pH 6.5, VAPOR DIFFUSION, temperature 293K