4EYD
Human Insulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Spacegroup name | H 3 |
Unit cell lengths | 80.830, 80.830, 33.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.410 - 1.471 |
R-factor | 0.1563 |
Rwork | 0.153 |
R-free | 0.22310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.415 | 14.049 | 1.550 |
High resolution limit [Å] | 1.471 | 4.650 | 1.471 |
Rmerge | 0.032 | 0.275 | |
Total number of observations | 2617 | 11505 | |
Number of reflections | 13635 | ||
<I/σ(I)> | 18.4 | 19.1 | 2.4 |
Completeness [%] | 99.3 | 95.4 | 100 |
Redundancy | 6 | 6.3 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Insunorm R, lot # I 550005), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |