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4EYD

Human Insulin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsLNLS BEAMLINE D03B-MX1
Synchrotron siteLNLS
BeamlineD03B-MX1
Temperature [K]293
Detector technologyCCD
DetectorMAR CCD 165 mm
Spacegroup nameH 3
Unit cell lengths80.830, 80.830, 33.160
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution40.410 - 1.471
R-factor0.1563
Rwork0.153
R-free0.22310
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.020
RMSD bond angle1.800
Data reduction softwareMOSFLM
Data scaling softwareSCALA (3.3.15)
Phasing softwarePHASER (2.1.4)
Refinement softwareREFMAC (5.5.0102)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]40.41514.0491.550
High resolution limit [Å]1.4714.6501.471
Rmerge0.0320.275
Total number of observations261711505
Number of reflections13635
<I/σ(I)>18.419.12.4
Completeness [%]99.395.4100
Redundancy66.35.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.52932 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Insunorm R, lot # I 550005), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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PDB entries from 2024-09-18

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