4EY9
Human Insulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Spacegroup name | H 3 |
Unit cell lengths | 80.780, 80.780, 33.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.390 - 1.471 |
R-factor | 0.1817 |
Rwork | 0.177 |
R-free | 0.25460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.061 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.390 | 13.463 | 1.550 |
High resolution limit [Å] | 1.471 | 4.650 | 1.471 |
Rmerge | 0.039 | 0.181 | |
Total number of observations | 1158 | 5351 | |
Number of reflections | 13402 | ||
<I/σ(I)> | 10.3 | 11.7 | 3.3 |
Completeness [%] | 97.7 | 90.1 | 99 |
Redundancy | 2.8 | 2.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Insunorm R, lot # I 550005), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |