4EY3
Crystal structure of solute binding protein of ABC transporter in complex with p-hydroxybenzoic acid
Replaces: 3T0NExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.816, 132.625, 130.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.220 |
R-factor | 0.1842 |
Rwork | 0.182 |
R-free | 0.23410 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.983 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.149 | 0.541 |
Number of reflections | 19925 | |
<I/σ(I)> | 16.3 | |
Completeness [%] | 98.5 | 96.3 |
Redundancy | 4.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1M SODIUM CITRATE, 20% ISO-PROPANOL,20% PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |