4EY1
Human Insulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Spacegroup name | H 3 |
Unit cell lengths | 80.860, 80.860, 33.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.430 - 1.471 |
R-factor | 0.1641 |
Rwork | 0.160 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.173 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.430 | 14.054 | 1.550 |
High resolution limit [Å] | 1.471 | 4.650 | 1.471 |
Rmerge | 0.027 | 0.225 | |
Total number of observations | 1312 | 5632 | |
Number of reflections | 13639 | ||
<I/σ(I)> | 13.7 | 21.7 | 3.1 |
Completeness [%] | 99.3 | 94.7 | 100 |
Redundancy | 3 | 3.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Insunorm R, lot # I 550005), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |