4EXW

The structure of DdrB from Deinococcus: a new fold for single-stranded DNA binding proteins.

Replaces:  3KDV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9798
Spacegroup name	P 32
Unit cell lengths	102.872, 102.872, 96.731
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.235 - 2.800
R-factor	0.2327
Rwork	0.231
R-free	0.25440
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.768
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.800	2.800
Rmerge	0.096	0.423
Number of reflections	27742
<I/σ(I)>		6.67
Completeness [%]	99.9	100
Redundancy	10.8	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1 M Sodium acetate anhydrous, 2.45 M Ammonium sulfate, 0.01 M Praseodymium(III) acetate hydrate., pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K