4EXV
Structure of Kluyveromyces lactis Hsv2p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-10-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 157.236, 157.236, 157.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.720 - 3.000 |
R-factor | 0.221 |
Rwork | 0.218 |
R-free | 0.27100 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.273 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 13828 |
Completeness [%] | 98.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 50 mM MES pH 6.2, 1.8 M Magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |