4EXT
Structure of polymerase-interacting domain of human Rev1 in complex with translesional synthesis polymerase zeta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Detector technology | CCD |
Detector | ADSC QUANTUM 270 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.699, 71.523, 84.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
Rwork | 0.224 |
R-free | 0.28510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.042 | 0.027 | 0.297 |
Number of reflections | 22498 | ||
<I/σ(I)> | 28.1 | ||
Completeness [%] | 96.0 | 98.7 | 80.9 |
Redundancy | 5.2 | 6.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 293 | 0.1M HEPES-Na (pH 7.5), 0.1M NH4-citrate tribasic (pH 7.0), 19-21% PEG 3350, EVAPORATION, temperature 293K |